The Needle Issue #23


Welcome to The Needle, a newsletter from Haystack Science to help you navigate the latest translational research, with a roundup of the latest news on preclinical biotech startups from around the world.

January was an upbeat month for biotech financing and dealmaking, although most of that activity was US-based. Funding highlights include the opening of the IPO market for companies with clinical assets like Aktis Oncology, Erasca, Agomab and Eikon, and $100+ million financing rounds for private biotechs with assets already in human testing (Tenpoint Therapeutics, AirNexus Therapeutics, Alveus Therapeutics, and Caldera Therapeutics). We counted five seed rounds and five series A rounds for earlier-stage preclinical builds covered by Haystack Science. Scientists from MatriSys Biosciences, PAM2L Biotechnologies, AVIL Therapeutics, Capstan Therapeutics (now part of Abbvie), Profluent Bio, Scribe Therapeutics, CorriXR Therapeutics, and NanoVation Therapeutics published research surrounding their assets. Strikingly, even startups in the graveyard field of therapeutic peptide cancer vaccines—Infinitopes and ErVimmune—were able to raise significant rounds. Elsewhere, the hangover following the bonanza of deals leading into JP Morgan was broken by Eli Lilly, which was busy forging partnerships with Repertoire Immune Medicines and Seamless Therapeutics, while it splashed out a whopping $1.2 billion for NLRP3 play Ventyx Bioscience. As usual, anything we missed in the biotech startup world, let us know (info@haystacksci.com).

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In our past issue, we took a look at all the financing deals that The Needle has covered since our inaugural issue. This week we turn our attention to last year’s deal making in the preclinical biotech space.

In 2025, preclinical dealmaking didn’t just slow — it polarized. Capital clustered around AI-enabled discovery, China-sourced assets, and in vivo CAR-T cell therapies, while entire therapeutic categories effectively disappeared from licensing activity. Based on the 131 publicly disclosed preclinical transactions in our sample, we reveal where early-stage risk capital is still flowing — and where it has quietly retreated.

Similar to the data we reported in our past newsletter, our analysis captures only publicly disclosed deals (partnerships, research collaborations, licenses, joint ventures, reverse mergers, equity investments and options) on business wires, industry news sites, and venture-fund sources. In the preclinical space, many deals are carried out in stealth, and companies in some important regions (like China) don’t use business wires or news sources traditionally available in the West. For these reasons, our estimates underestimate the true level of early-stage preclinical dealmaking.

In total, we tracked 131 preclinical deals over the year, of which 42 were licensing deals, 64 were strategic partnerships/collaborations and 14 were mergers and acquisitions (M&As). In keeping with early stage’s exploratory nature, the importance of stealth, and the non-compensatory nature of much of the work done, over half of the publicly announced strategic partnerships (35 deals; 55%) had no terms disclosed. As one would expect, a smaller proportion of the licensing deals failed to provide terms, but even for this category, 8 of the 48 transactions (17%) didn’t give financial details. Four of the 14 M&As that we tracked also made no mention of deal terms.

US-headquartered companies continue to dominate the dealmaking landscape, whether it is research collaborations, licensing or trade sales. One reason for the dominance of companies in the US — and the UK, which is second in deal activity — is likely simple math; a greater number of companies are financed and built in these countries compared with the rest of the globe (see The Needle Issue #22).

Strategic partnerships in 2025 favored platforms over products — and Western biotechs over Asian peers.

The 64 strategic partnerships we tracked had upfront payments that ranged from $5 million to $110 million, but the median ($35.5 million) underscores how concentrated value remains in a handful of outlier platform deals.

US companies accounted for 37 of the 64 deals (58%). Three notable partnering big-ticket deals involved biotechs splashing out large sums on preclinical collaborations, with the payers showing interest in branching out into new therapeutic modalities: last May, CRISPR Therapeutics (San Diego, CA) pivoted from gene editing to siRNA, paying $95 million to Sirius Therapeutics (Shanghai, China) to co-develop a long-acting siRNA designed to selectively inhibit Factor XI for thrombosis; in December, Regeneron Pharmaceuticals (Tarrytown, NY) spent $150 million (and made an equity investment) to jointly develop Tessera Therapeutics’ (Somerville, MA) target-primed reverse transcription therapy (TSRA-196), which uses lipid nanoparticles (LNPs) to deliver RNAs encoding an engineered reverse transcriptase (‘gene writer’), writer-recognition motifs, and a SERPINA1 template to correct a mutation in alpha 1 antitrypsin deficiency; and later the same month, peptide developer Zealand Pharma (Søborg, Denmark) announced a transaction with OTR Therapeutics (Shanghai, China), paying $20 million upfront for small-molecule programs centered around validated targets of Zealand’s franchise in cardio-metabolic disease.

For obvious reasons, target discovery and drug screening comprise about a third of collaborations and partnership agreements, but do not figure much in licensing and M&A. Mentions of machine learning in partnering deals (18.2% of 2025’s deals, with several in the top 10 grossing set) suggest large-language and other models are an increasingly established facet of preclinical development. Neurodegenerative disorders garnered the second largest number of partnering transactions in our 2025 sample. And, with all the noise around GLP-1s and other incretins, metabolic disease and obesity were the focus of 11% of deals.

Perhaps the most counterintuitive finding in the partnership data is the near-total absence of China-headquartered companies — despite their dominance in preclinical licensing. This may reflect geopolitical friction, IP risk tolerance or a Western preference for control in collaborations. Alternatively, the absence may reflect the limitations of Haystack’s methodology for collecting data. Certainly, the partnership data contrasts starkly with our licensing data, which show Chinese assets performing so well that they are biting at the heels of US companies and running far ahead of UK companies. In contrast, for strategic partnerships, it was UK-, and South Korea-based firms that were most prominent behind the US (15%, and 7% of dealmaking, respectively).

For licensing, the shift to Asia seen in later parts of the biotech pipeline is also manifest in the preclinical space.

Chinese companies were involved in nearly a quarter of all the licensing deals made last year, clinching 11 out of the 48 deals we tracked. This interest in early-stage Chinese assets mirrors last year’s banner deals for later-stage assets, such as Pfizer’s ex-China rights acquisition of 3SBio’s (Shenyang, China) PD-1 x VEGF bispecific antibody for $1.25 billion, or GSK’s $1.10 billion acquisition of Jiangsu Hengrui’s (Lianyungang, China) phosphodiesterase 3/4 inhibitor and oncology portfolio. Overall, deals seeking access to assets from Asian biotechs (companies based in China, South Korea, Singapore and Taiwan) comprised 33% of all preclinical licensing transactions in our sample.

Looking at the preclinical licensing as a whole, upfront amounts ranged from $0.7 million to $700 million, with a median value of $35 million. Most deals centered around cancer, followed by autoimmune, neurodegenerative and metabolic diseases.

What was perhaps most surprising is that we didn’t see any licenses for preclinical assets in the cardiovascular space, suggesting that the interest of a few years ago has somewhat diminished (although assets for heart disease still made up 4% of partnering agreements). Notably absent from preclinical licensing in 2025: cardiovascular, pulmonary, skeletomuscular, hepatic, pain, psychiatry, women’s health, sleep, hearing, and stroke. This pattern perhaps reinforces the industry’s retrenchment toward genetically anchored, biologically de-risked indications. Together, these licensing gaps underscore a 10-year low in early-stage risk appetite outside traditional blockbuster categories.

The top 10 licensing deals from last year are listed in the Table below. Of this elite tier of top-grossing deals, cancer and autoimmune comprised the lion’s share (70%), with neurodegenerative, neurodevelopmental, metabolic, and ophthalmic disease all represented. Only two of the top 10 deals involved traditional small molecules (with one additional license for a molecular glue), whereas biologics accounted for seven. While small molecules still comprise the biggest chunk of licensing activity (18.9%), deals trended toward bispecific and multispecific antibodies for cancer immunology and autoimmune indications — and biopharma was prepared to pay: Of the 8 licensing transactions for multispecifics in our sample, IGI Therapeutics’ (New York, NY) deal with Abbvie, and CDR Life’s (Zurich, Switzerland) agreement with Boehringer Ingelheim, ended among the top 10 grossing deals of the year.

Which leads us to mergers. Overall, we tracked 14 M&A deals last year in the preclinical space. According to Dealforma data presented at JP Morgan, private biopharma accounted for just over 55% of merger activity in 2025 on par with previous years. In the Haystack data, 12 of the 14 acquisitions for preclinical programs were for US-based private companies, reinforcing the historical trend of American biotechs outperforming those in the rest of the world in terms of negotiating successful exits for their investors.

The biggest story in early-stage mergers from last year, though, was biopharma’s ravenous appetite for in vivo CAR-T cell therapy, with Capstan, Orbital and Interius comprising 3 of the 14 acquisitions recorded by Haystack, all of which ranked among the top 5 highest upfront payments. As our sampling commenced in April 2025, we missed another deal: AstraZeneca’s acquisition of lentiviral in vivo CAR-T therapy developer Esobiotec, originally announced in March 2025 with an upfront of $425 million. All in all, in vivo CAR-T therapies claimed 4 of the top 5 acquisitions last year.

The use of lipid nanoparticles (LNPs) in many of these in vivo CAR-T platforms (Orbital, Aera Therapeutics, Stylus Medicine, MagicRNA, Orna Therapeutics, Byterna Therapeutics and Strand Therapeutics) and elsewhere (Tessera, Starna Therapeutics, Nanovation Therapeutics, United Immunity, Genevant Sciences, Pantherna Therapeutics, eTheRNA Immunotherapies, and Beam Therapeutics) also underlies a continuing theme of investment and dealmaking around drug delivery platforms.

Apart from LNPs, several drug delivery deals also centered around antibody shuttles that can take biologics and siRNAs across the blood–brain barrier into the CNS. These included Manifold Bio/Roche, Vect-Horus/Secarna, Ophidion/Neuronasal, JCR/Acumen and Denali/Royalty Pharma. This year will see more of these shuttles enter clinical testing, with Alector’s transferrin shuttle AL137, a subcutaneous anti-amyloid beta antibody, slated for an IND submission.

In sum, the preclinical dealscape in 2025 reveals an industry willing to fund innovation — but only when paired with platform leverage, delivery, or late-stage optionality. As Haystack tracks dealmaking through 2026, the key question will not be whether capital returns to early-stage biotech, but whether it broadens beyond today’s narrow set of ‘acceptable’ risks. We look forward to tracking deals throughout 2026 and identifying new emerging trends in biotech deals.

Translational papers: Best of the rest

Target biology

Peripheral cancer attenuates amyloid pathology in Alzheimer’s disease via cystatin-c activation of TREM2 | Cell

Identification of Or5v1/Olfr110 as an oxylipin receptor and anti-obesity target | Cell

Reactive oligodendrocytes promote glioblastoma progression through CCL5/CCR5-mediated glioma stem cell maintenance | Neuron

MatriSys Biosciences reports that dermal fibroblasts respond to interleukin-4 and 13 and promote T-cell recruitment in atopic dermatitis | JCI

SREBP-1 increases glucose uptake to promote tumor resistance to lysosome inhibition | Science Translational Medicine

Targeted inhibition of FBXL2 confers susceptibility of HER2-negative breast cancer to trastuzumab deruxtecan | Nature Cancer

Vascular smooth muscle cell-derived KIF13B protects against atherosclerosis: evidence from humans and mice | JCI

CB1R and GlyT2 interaction in spinal glycinergic circuits drives neuropathic mechanical pain | Neuron

Repression of RIPK1 kinase by INPP5D inhibits expression of diverse proinflammatory mediators and late-onset Alzheimer’s disease risk factors | Immunity

Gut microbiota-derived isovaleric acid alleviates atrial fibrillation by suppressing GSDME-dependent pyroptosis | Cell Metabolism

Sine oculis homeobox 1 drives endothelial dysfunction in preclinical pulmonary arterial hypertension | Science Translational Medicine

Overexpression of the ERG oncogene in prostate cancer identifies candidates for PARP inhibitor-based radiosensitization | JCI

ZFTA–RELA ependymomas make itaconate to epigenetically drive fusion expression | Nature

Activated ATF6α is a hepatic tumour driver restricting immunosurveillance | Nature

Target validation

Shenzhen’s PAM2L Biotechnologies discloses engineered bacteria that secrete CP43K and TFF3 to treat inflammatory bowel disease | Nature Biotechnology

Selective targeting of NRF2-high pancreatic ductal adenocarcinoma with an NQO1-activatable prodrug | PNAS

Capstan Therapeutics (now part of AbbVie) and collaborators show that anti-FAP CAR T cells produced in vivo reduce fibrosis and restore liver homeostasis in steatohepatitis | Science Translational Medicine

In silico screening and preclinical validation identify bavisant as a therapeutic candidate for multiple sclerosis | Science Translational Medicine

AVIL Therapeutics reports a first-in-class small-molecule inhibitor of AVIL with preclinical efficacy in glioblastoma | Science Translational Medicine

Dual targeting of SLC25A51 and succinate dehydrogenase selectively depletes mitochondrial NAD+ to eradicate KRAS-driven AML | Cell Metabolism

Targeting modulated vascular smooth muscle cells in atherosclerosis via FAP-directed immunotherapy | Science

Next-generation anti-DLL3 radiopharmaceuticals targeting high-grade neuroendocrine lung and prostate cancers | PNAS

Small-molecule inhibition of YTHDC1 as a strategy against acute myeloid leukemia in mouse models | Science Translational Medicine

Cancer immunotherapy

Bioresponsive immunomodulator nanocomplex for selective immunoengineering in metastatic lymph nodes | PNAS

Tumor-antigen-independent targeting of solid tumors by armored macrophage-directed anti-TREM2 CAR T cells | Cancer Cell

Armored macrophage-targeted CAR-T cells reset and reprogram the tumor microenvironment and control metastatic cancer growth | Cancer Cell

IGSF11-VISTA is a critical and targetable immune checkpoint axis in diffuse midline glioma | Cancer Cell

Oligodendrocyte transcription factor 2 orchestrates glioblastoma immune evasion by suppressing CXCL10 and CD8+ T cell activation | JCI

Targeting Pim2 improves antitumor immunity through promoting effector function and persistence of CD8 T cells | JCI

MAP4K2 suppresses antitumor immunity in a pancreatic cancer model by promoting Treg differentiation | JCI

Posttranscriptional regulation of PD-1 by PRMT5/WDR77 complex shapes T cell effector function and antitumor immunity | JCI

Proanthocyanidins enhance antitumor immunity by promoting ubiquitin-proteasomal PD-L1 degradation via stabilization of LKB1 and SYVN1 | JCI

Chimeric antigen receptor T cells against the IGHV4-34 B cell receptor specifically eliminate neoplastic and autoimmune B cells | Science Translational Medicine

Enhancing CAR- and TCR-mediated targeting of cancer via an immune synapse-stabilizing receptor | Nature Communications

Platforms, delivery, editing

An engineered UGA suppressor tRNA gene for disease-agnostic AAV delivery | Nature Biotechnology

De novo design of potent CRISPR–Cas13 inhibitors | Nature Chemical Biology

An organ-conformal, kirigami-structured bioelectronic patch for precise intracellular delivery | Cell

AAVLINK: A potent DNA-recombination method for large cargo delivery in gene therapy | Cell

Crystallized colony-stimulating factor-1 receptor inhibitor protects immunoisolated allo but not xeno transplants in primates, according to research from Sigilon Therapeutics (now part of Lilly) and their collaborators | Science Translational Medicine

A team from Profluent Bio and collaborators report on a way to customize CRISPR–Cas PAM specificity with protein language models | Nature Biotechnology

Programmable genome editing in human cells using RNA-guided bridge recombinases | Science

Scibe Therapeutics achieves durable LDL-C lowering in primates using STX-1150, its CRISPR-CasX-based epigenetic repressor platform targeting ATRX-DNMT3A-DNMT3L (ADD) domain to unmodified H3K4 around PSCK9 for reducing cardiovascular risk | BioRxiv

CorriXR cites target choice and exon skipping as factors regulating CRISPR-Cas9 directed gene editing of transcription factor NRF2 in head/neck and esophageal cancer cells | Mol Therapy Oncology

NanoVation Therapeutics’ topically applied lipid nanoparticle allows in situ cytosine (eTd) base editing of congenital ichthyosis-causing mutations in human skin models | Cell Stem Cell

Startup news

January saw more money being made available for biotech ventures:

Sante Ventures exceeds $300 million target with Fund V for innovative biotech, medical technology, and digital health

Scottish-based Epidarex Capital closes $145 million Fund IV focused on early-stage UK-US life science investing.

Yosemite plans new $350 million Fund II aimed at investing in cancer research

However, it wasn’t all good news:

European venture fund Gimv exits life science investments to focus on Consumer, Healthcare, Smart Industries and Sustainable Cities.

At the state level, there were some notable announcements related to funding and training:

Texas’ CPRIT announces $76.92 million in grants last year for development research in companies, including Allterum Therapeutics, Eisbach Bio, Emtora Biosciences, Marker Therapeutics, Metaclipse Therapeutics, OncoResponse, Orphagen Pharmaceuticals, Telos Biotechnology, and Ypsilon Therapeutics

MassBio, in partnership with The Termeer Institute, launches Termeer Ascend: Leadership in Practice, a values-driven leadership development program designed to prepare senior-level biotech professionals for C-suite roles

Virginia State Authority’s Activation Capital updates Frontier Biohealth executive training programs to make them more flexible to needs of life science entrepreneurs

Preclinical financings

Preclinical deals

Stay in touch

We hope you enjoyed this issue of The Needle and hit the button below to receive forthcoming issues into your inbox

If you’re interested in commercializing your science, get in touch. We can help you figure out the next steps for your startup’s translational research program and connect you with the right investor. Follow us on X, BlueSky and LinkedIn. Please send feedback; we’d love to hear from you (info@haystacksci.com).

Until next week,

Juan Carlos and Andy

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